Affiliation:
1. Centre National de la Recherche Scientifique, CERCOA, 94320 Thiais, France
Abstract
Important discrepancies in isoelectric points (pI) of β-lactamases were observed depending on the experimental procedure used for their determination: isoelectric focusing (IEF) in sucrose density gradients or analytical IEF in thin-layer polyacrylamide gels (PA). The variations, negligable in the case of TEM-like β-lactamases, appeared to be important in the case of cephalosporinases and are related to an artifact which appears in PA-IEF. This has been clearly shown with β-lactamase preparations from the following bacterial strains:
Pseudomonas aeruginosa
NCTC 8203 (pI = 8.7),
P. aeruginosa
NCTC 10701 (pI = 9.4), and
Proteus morganii
NCTC 235 (pI = 8.3). The data previously obtained by PA-IEF were much lower.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference12 articles.
1. Arbuthnott J. P. and J. A. Beeley (ed.). 1975. Isoelectric focusing. Butterworth London.
2. Multiplicite des ,B lactamases: un probleme d'isoencymes;Labia R.;C. R. Acad. Sci. Ser. D,1976
3. Cephalosporinases constitutives de Escherichia coli;Labia R.;Ann. Microbiol. (Inst. Pasteur),1976
4. Etude cinetique d'une nouvelle cephalosporinase de Pseudomonas aeruginosa;Labia R.;Biochimie,1976
5. Beta-lactamases produced by a Pseudomonas aeruginosa strain highly resistant to carbenicillin;Labia R.;Antimicrob. Agents Chemother.,1977
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