Radioimmunoassay for Measurement of Gentamicin in Blood

Author:

Mahon W. A.1,Ezer J.1,Wilson T. W.1

Affiliation:

1. The Division of Clinical Pharmacology, Department of Medicine, Toronto General Hospital, Toronto, Ontario, Canada

Abstract

Gentamicin toxicity has been shown to be related to high concentrations in serum. Because there is a narrow range between its therapeutic and toxic levels, serial monitoring of gentamicin is the most reliable method of guiding therapy. Microbiological assays commonly in use do not afford the desired speed and accuracy, and results may be difficult to interpret in the presence of other antimicrobials. Hence, a rapid, sensitive, and highly specific radioimmunoassay for measurement of gentamicin in serum has been developed. Antibody to gentamicin was raised in rabbits by using a gentamicin-albumin conjugate. Tritiated gentamicin (specific activity 1.0 Ci/mM) competes with unlabeled gentamicin for binding sites on the antibody. Dextran-coated charcoal separates the unbound from antibody-bound gentamicin. Serum levels of gentamicin are determined by comparison with a standard curve. This method can detect concentrations as low as 0.01 μg/ml. Results of a 24-tube run can be obtained in 1 h, thus allowing modification of gentamicin dosage to advantage.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference17 articles.

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3. Gentamicin therapy in renal failure: a nomogram for dosage;Chan R. A.;Ann. Int. Med.,1972

4. Correlation of serum creatinine concentration and gentamicin half-life;Cutler R. E.;J. Amer. Med. Ass.,1972

5. On the mechanism of carboxyl condensations of carbodiimides;Doleschall G.;Tetrahedron Lett.,1963

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