Evaluation of a Wipe Surface Sample Method for Collection of Bacillus Spores from Nonporous Surfaces-Reference-Cited by-同舟云学术

Evaluation of a Wipe Surface Sample Method for Collection of Bacillus Spores from Nonporous Surfaces

Published:2007-02 Issue:3 Volume:73 Page:706-710
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Brown Gary S.¹,Betty Rita G.²,Brockmann John E.³,Lucero Daniel A.³,Souza Caroline A.¹,Walsh Kathryn S.¹,Boucher Raymond M.⁴,Tezak Mathew⁵,Wilson Mollye C.⁵,Rudolph Todd⁶

Affiliation:

1. Sandia National Laboratories, P.O. Box 5800, MS0734, Albuquerque, New Mexico 87185

2. Sandia National Laboratories, P.O. Box 5800, MS0115, Albuquerque, New Mexico 87185

3. Sandia National Laboratories, P.O. Box 5800, MS0836, Albuquerque, New Mexico 87185

4. Orion International Technologies, P.O. Box 5800, MS0734, Albuquerque, New Mexico 87185

5. American Staff Augmentation Providers, P.O. Box 5800, MS0734, Albuquerque, New Mexico 87185

6. Tactical Staffing Resources, P.O. Box 5800, MS0836, Albuquerque, New Mexico 87185

Abstract

ABSTRACT Polyester-rayon blend wipes were evaluated for efficiency of extraction and recovery of powdered Bacillus atrophaeus spores from stainless steel and painted wallboard surfaces. Method limits of detection were also estimated for both surfaces. The observed mean efficiency of polyester-rayon blend wipe recovery from stainless steel was 0.35 with a standard deviation of ±0.12, and for painted wallboard it was 0.29 with a standard deviation of ±0.15. Evaluation of a sonication extraction method for the polyester-rayon blend wipes produced a mean extraction efficiency of 0.93 with a standard deviation of ±0.09. Wipe recovery quantitative limits of detection were estimated at 90 CFU per unit of stainless steel sample area and 105 CFU per unit of painted wallboard sample area. The method recovery efficiency and limits of detection established in this work provide useful guidance for the planning of incident response environmental sampling following the release of a biological agent such as Bacillus anthracis .

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01082-06

Reference22 articles.

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