Sensitive Method for Detection of Human Herpesviruses 6 and 7 in Saliva Collected in Field Studies-Reference-Cited by-同舟云学术

Sensitive Method for Detection of Human Herpesviruses 6 and 7 in Saliva Collected in Field Studies

Published:2000-05 Issue:5 Volume:38 Page:1981-1983
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Zerr Danielle M.¹,Huang Meei-Li²,Corey Lawrence²³,Erickson Matthew³,Parker Heather L.³,Frenkel Lisa M.¹²

Affiliation:

1. Departments of Pediatrics1 and

2. Laboratory Medicine,2 University of Washington, and

3. Department of Infectious Diseases, Fred Hutchinson Cancer Research Center,3 Seattle, Washington

Abstract

ABSTRACT To facilitate studies of the epidemiology and natural history of human herpesviruses 6 and 7 in infants, a practical method for collecting and quantifying the DNA of these viruses was developed. Saliva was collected using small strips of filter paper, and virus was detected using a real-time quantitative fluorescent-probe PCR assay. The sensitivity and specificity of this method even after prolonged drying of the specimens compared favorably to those of our traditional method of collecting and assaying saliva.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.38.5.1981-1983.2000

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