Molecular Analysis of the Enterobacter sakazakii O-Antigen Gene Locus-Reference-Cited by-同舟云学术

Molecular Analysis of the Enterobacter sakazakii O-Antigen Gene Locus

Published:2008-06-15 Issue:12 Volume:74 Page:3783-3794
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Mullane N.¹,O'Gaora P.²,Nally J. E.³,Iversen C.⁴,Whyte P.¹,Wall P. G.⁵,Fanning S.¹

Affiliation:

1. Centres for Food Safety and Food-borne Zoonomics, UCD Veterinary Sciences Centre

2. Conway Institute of Biomolecular & Biomedical Research

3. School of Agriculture, Food Science & Veterinary Medicine

4. Institute for Food Safety & Hygiene, Vetsuisse Faculty, University of Zurich, CH-8057 Zurich, Switzerland

5. School of Public Health & Population Sciences, University College Dublin, Belfield, Dublin 4, Ireland

Abstract

ABSTRACT Nucleotide polymorphism associated with the O-antigen-encoding locus, rfb , in Enterobacter sakazakii was determined by PCR-restriction fragment length polymorphism analysis. Based on the analysis of these DNA profiles, 12 unique banding patterns were detected among a collection of 62 strains from diverse origins. Two common profiles were identified and were designated serotypes O:1 and O:2. DNA sequencing of the 12,500-bp region flanked by galF and gnd identified 11 open reading frames, all with the same transcriptional direction. Analysis of the proximal region of both sequences demonstrated remarkable heterogeneity. A PCR assay targeting genes specific for the two prominent serotypes was developed and applied for the identification of these strains recovered from food, environmental, and clinical samples.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02302-07

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