DNA Fingerprinting of Single Colonies of Helicobacter pylori from Gastric Cancer Patients Suggests Infection with a Single Predominant Strain

Author:

Miehlke Stephan1,Thomas Rachel2,Guiterrez Oscar3,Graham David Y.2,Go Mae F.2

Affiliation:

1. Medical Department I, Technical University Hospital, Dresden, Germany1;

2. Department of Medicine, Veterans Affairs Medical Center, Baylor College of Medicine, Houston, Texas2; and

3. University Hospital San Juan de Dios, Bogota, Colombia3

Abstract

ABSTRACT In each of six gastric cancer patients, repetitive extragenic palindromic PCR DNA fingerprints of 18 single colonies of Helicobacter pylori from the gastric antrum, corpus, and cardia were identical and matched that of the parental isolate. In three additional gastric cancer patients, 17 of 18 single-colony DNA fingerprints were identical to each other and to the DNA fingerprint of the corresponding parental isolate.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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1. Helicobacter pylori;Current Topics in Microbiology and Immunology;2021

2. Distinguishing Pathovars from Nonpathovars: Helicobacter pylori;Molecular Epidemiology of Infectious Diseases;2014-04-30

3. In Vivo Adaptation to the Host;Helicobacter pylori;2014-04-09

4. Heterogeneity and Subtyping;Helicobacter pylori;2014-04-09

5. Clonality Analysis ofHelicobacter pyloriin Patients Isolated from Several Biopsy Specimens and Gastric Juice in a Japanese Urban Population by Random Amplified Polymorphic DNA Fingerprinting;Gastroenterology Research and Practice;2013

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