Affiliation:
1. Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, London NW9 5HT, United Kingdom
Abstract
ABSTRACT
A PCR-based study of the incidence of enteropathogenic campylobacter infection in humans was done on the basis of a detection and identification algorithm consisting of screening PCRs and species identification by PCR-enzyme-linked immunosorbent assay. This was applied to DNA extracted from 3,738 fecal samples from patients with sporadic cases of acute gastroenteritis, submitted by seven regional Public Health Laboratories in England and Wales over a 2-year period. The sending laboratories had cultured “
Campylobacter
spp.” from 464 samples. The PCR methodologies detected 492
Campylobacter
-positive samples, and the combination of culture and PCR yielded 543
Campylobacter
-positive samples. There was identity (overlap) for 413 samples, but 79 PCR-positive samples were culture negative, and 51 culture-positive samples were PCR negative. While there was no statistically significant difference between PCR and culture in detection of
C. jejuni-C. coli
(PCR, 478 samples; culture, 461 samples), PCR provided unique data about mixed infections and non-
C. jejuni
and non-
C. coli
campylobacters. Mixed infections with
C. jejuni
and
C. coli
were found in 19 samples, and mixed infection with
C. jejuni
and
C. upsaliensis
was found in one sample; this was not apparent from culture. Eleven cases of gastroenteritis were attributed to
C. upsaliensis
by PCR, three cases were attributed to
C. hyointestinalis
, and one case was attributed to
C. lari
. This represents the highest incidence of
C. hyointestinalis
yet reported from human gastroenteritis, while the low incidence of
C. lari
suggests that it is less important in this context.
Publisher
American Society for Microbiology
Cited by
88 articles.
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