Affiliation:
1. Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115
Abstract
Direct assay for deoxyribonucleic acid polymerase II in extracts has been used to screen recombinants for the
polB
allele in Hfr × F
−
crosses, F-ductants in episome transfer, and transductants in generalized transduction by phage P1. The
polB
gene is located at 2 min on the
Escherichia coli
linkage map; it is 39 to 64% co-transducible with
leu
. A mutant,
E. coli polA1 polB100 polC
(ts), deficient in deoxyribonucleic acid polymerases I and II and having a thermolabile deoxyribonucleic acid polymerase III, has been prepared by the P1-mediated cross: P1 (HMS85
polB100
) ×
E. coli
BT1026
polA1 polC
(ts)
leu
−
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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