Specific Inhibitors of Ammonia Oxidation in Nitrosomonas

Author:

Hooper Alan B.1,Terry Kathleen R.1

Affiliation:

1. Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101

Abstract

The following compounds or treatments have been shown to inhibit the oxidation of ammonia, but not the oxidation of hydroxylamine in cells of Nitrosomonas : (i) metal-binding agents such as allylthiourea or potassium cyanide; (ii) compounds such as SKF 525 which interact with cytochrome P-450 of mammalian microsomes; (iii) carbon monoxide; (iv) inhibitors of catalase, peroxidase, and amine oxidases such as thiosemicarbazide, ethylxanthate, and iproniazid, respectively; (v) uncouplers of oxidative phosphorylation such as m -chlorocarbonylcyanidephenylhydrazone; (vi) electron acceptors such as phenazine methosulfate; (vii) compounds such as methanol or N 2 O which react with free radicals; and (viii) illumination with 420 lux (5,000 foot candles) of light.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference23 articles.

1. Generation of reducing power in chemosynthesis. II. Energy-linked reduction of pyridine nucleotide in the chemoautotroph Nitrosomonas europaea;Aleem M. I. H.;Biochim. Biophys. Acta,1966

2. Studies on the oxidation of ammonia to hydroxylamine by Nitrosomonas europaea;Anderson J. H.;Biochem. J.,1964

3. Purification and properties of peroxidase from Nitrosomonas europaea;Anderson J. R.;J. Bacteriol.,1968

4. The effect of 2-chloro-6-(trichloromethyl)pyridine on the chemoautotrophic metabolism of nitrifying bacteria. I. Ammonia and hydroxylamine oxidation by Nitrosomonas. Antonie van Leeuwenhoek J;Campbell N. E. R.;Microbiol. Serol.,1965

5. Preliminarv characterization of a variant CO-binding heme protein from;Erickson R. H.;Nitrosomonas. Biochim. Biophys.,1972

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