Intra-Assay Performance Characteristics of Five Assays for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma

Author:

Lin Hsiang Ju1,Pedneault Louise2,Hollinger F. Blaine1

Affiliation:

1. Division of Molecular Virology, Baylor College of Medicine, Houston, Texas,1 and

2. Antiviral Clinical Research Department, Bristol-Myers Squibb Pharmaceutical Research Institute, Wallingford, Connecticut2

Abstract

ABSTRACT Three kits (Roche AMPLICOR human immunodeficiency virus type 1 [HIV-1] Monitor, Chiron enhanced-sensitivity bDNA, and Organon Teknika NASBA HIV-1 QT) and two in-house assays (from National Genetics Institute and Baylor College of Medicine) were compared with a blinded panel. The results were evaluated as to intra-assay sensitivity, precision, and ability to detect differences in a dilution series.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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1. Laboratory Diagnosis of HIV-1 Infections: State of the Art;Advanced Techniques in Diagnostic Microbiology;2018

2. Human Immunodeficiency Virus;Clinical Virology;2016-03-07

3. Principles and Procedures of Human Immunodeficiency Virus Diagnosis;Manual of Molecular and Clinical Laboratory Immunology;2016-01-27

4. Human Immunodeficiency Viruses;Manual of Clinical Microbiology;2015-05-26

5. Monitoring the Quality of HIV-1 Viral Load Testing through a Proficiency Testing Program Using Dried Tube Specimens in Resource-Limited Settings;Journal of Clinical Microbiology;2015-01-21

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