Author:
Murphy T F,Bartos L C,Campagnari A A,Nelson M B,Apicella M A
Abstract
The purpose of this study was to characterize the degree of antigenic heterogeneity or conservation of a 16,600-dalton outer membrane protein (P6) among strains of nontypable Haemophilus influenzae. Immunization of rabbits with P6 isolated from individual strains resulted in antibody to P6 of all 25 strains tested. The titers of antibody in the sera were similar among the strains. Whole organisms of two strains were used to immunize rabbits, and antibodies were produced to P6 of all strains tested. Monoclonal antibodies developed to P6 from mice immunized with whole cells of three different strains recognized determinants on P6 of all 25 strains tested. Finally, pooled normal human serum contained antibodies to P6 of all 25 strains assayed. These studies indicate that P6 is a highly conserved antigen on the outer membrane of nontypable H. influenzae.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference19 articles.
1. Antigenic heterogeneity of lipid A of Haemophilus influenzae;Apicelia M. A.;Infect. Immun.,1985
2. Subtyping isolates of Haemophilus influenzae type b by outermembrane protein profiles;Barenkamp S. J.;J. Infect. Dis.,1981
3. Nontypeable Haemophilus influenzae in the elderly;Berk S. L.;Arch. Intern. Med.,1982
4. Bluestone C. D. and J. 0. Klein. 1983. Otitis media with effusion atelectasis and eustachian tube dysfunction p.356. In C. D. Bluestone and S. E. Stool (ed.) Pediatric otolaryngology. W. B. Saunders Co. Philadelphia.
5. Sinusitis of the maxillary atrium;Evans F. O.;N. Engl. J. Med.,1975
Cited by
89 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献