Minimal Requirements for Exponential Growth of Lactococcus lactis-Reference-Cited by-同舟云学术

Minimal Requirements for Exponential Growth of Lactococcus lactis

Published:1993-12 Issue:12 Volume:59 Page:4363-4366
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Jensen Peter Ruhdal¹,Hammer Karin¹

Affiliation:

1. Department of Microbiology, Technical University of Denmark, DK-2800 Lyngby, Denmark, 2 and Division of Molecular Biology, Netherlands Cancer Institute H5, NL-1066-CX, Amsterdam, The Netherlands

Abstract

A minimal growth medium containing glucose, acetate, vitamins, and eight amino acids allowed for growth of Lactococcus lactis subsp. lactis , with a specific growth rate in batch culture of μ = 0.3 h -1 . With 19 amino acids added, the growth rate increased to μ = 0.7 h -1 and the exponential growth phase proceeded until high cell concentrations were reached. We show that morpholinepropanesulfonic acid (MOPS) is a suitable buffer for L. lactis and may be applied in high concentrations.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.59.12.4363-4366.1993

Reference14 articles.

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