Comparison of the Digene Hybrid Capture System Cytomegalovirus (CMV) DNA (Version 2.0), Roche CMV UL54 Analyte-Specific Reagent, and QIAGEN RealArt CMV LightCycler PCR Reagent Tests Using AcroMetrix OptiQuant CMV DNA Quantification Panels and Specimens from Allogeneic-Stem-Cell Transplant Recipients-Reference-Cited by-同舟云学术

Comparison of the Digene Hybrid Capture System Cytomegalovirus (CMV) DNA (Version 2.0), Roche CMV UL54 Analyte-Specific Reagent, and QIAGEN RealArt CMV LightCycler PCR Reagent Tests Using AcroMetrix OptiQuant CMV DNA Quantification Panels and Specimens from Allogeneic-Stem-Cell Transplant Recipients

Published:2007-06 Issue:6 Volume:45 Page:1972-1973
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hanson Kimberly E.¹²,Reller L. Barth¹²,Kurtzberg Joanne³,Horwitz Mitchell⁴,Long Gwynn⁴,Alexander Barbara D.¹²

Affiliation:

1. Division of Infectious Diseases and International Health

2. Clinical Microbiology Laboratory, Department of Pathology, Duke University Medical Center, Durham, North Carolina

3. Pediatric Blood and Marrow Transplant Program, Department of Pediatrics

4. Division of Cellular Therapy, Department of Medicine

Abstract

ABSTRACT The Digene Hybrid Capture system cytomegalovirus (CMV) DNA (version 2.0), Roche CMV UL54 analyte-specific reagent, and QIAGEN RealArt CMV LightCycler PCR reagent tests were compared using whole-virus standards and plasma specimens collected from allogeneic-stem-cell transplant recipients. PCR assays showed better speed, sensitivity, and specificity.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02515-06

Reference3 articles.

1. Multicenter Comparison of the Digene Hybrid Capture CMV DNA Assay (Version 2.0), the pp65 Antigenemia Assay, and Cell Culture for Detection of Cytomegalovirus Viremia

2. Razonable, R. R., R. A. Brown, J. Wilson, C. Groettum, W. Kremers, M. Espy, T. F. Smith, and C. V. Paya. 2002. The clinical use of various blood compartments for cytomegalovirus (CMV) DNA quantitation in transplant recipients with CMV disease. Transplantation73:968-973.

3. High Variability between Results of Different In-House Tests for Cytomegalovirus (CMV) Monitoring and a Standardized Quantitative Plasma CMV PCR Assay

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