Use of Blue Agar CAS Assay for Siderophore Detection-Reference-Cited by-同舟云学术

Use of Blue Agar CAS Assay for Siderophore Detection

Published:2011-01 Issue:1 Volume:12 Page:51-53
ISSN:1935-7877
Container-title:Journal of Microbiology & Biology Education
language:en
Short-container-title:J Microbiol Biol Educ.

Author:

Louden Brian C.¹,Haarmann Daniel¹,Lynne Aaron M.¹

Affiliation:

1. Department of Biological Sciences, Sam Houston State University, Huntsville, TX 77341

Abstract

Schwyn and Neiland developed a universal siderophore assay using chrome azurol S(CAS) and hexadecyltrimethylammonium bromide (HDTMA) as indicators. In Schwyn and Neiland’s original paper, the procedure given for making CAS agar is written in general terms and can be difficult to follow, especially for an individual who has limited experience making more complex media. Here, we give a step-by-step protocol for making the CAS agar plates, and we discuss how this media can be incorporated in a comprehensive project in a microbiology lab course for biology majors.

Publisher

American Society for Microbiology

Subject

General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,Education

Link

https://journals.asm.org/doi/pdf/10.1128/jmbe.v12i1.249

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