Affiliation:
1. Centro de Citricultura Sylvio Moreira, Instituto Agronômico, CEP 13490-970, Cordeirópolis, São Paulo, Brazil
Abstract
ABSTRACT
Short sequence repeats (SSRs) with a potential variable number of tandem repeat (VNTR) loci were identified in the genome of the citrus pathogen
Xylella fastidiosa
and used for typing studies. Although mono- and dinucleotide repeats were absent, we found several intermediate-length 7-, 8-, and 9-nucleotide repeats, which we examined for allelic polymorphisms using PCR. Five genuine VNTR loci were highly polymorphic within a set of 27
X. fastidiosa
strains from different hosts. The highest average Nei's measure of genetic diversity (
H
) estimated for VNTR loci was 0.51, compared to 0.17 derived from randomly amplified polymorphic DNA (RAPD) analysis. For citrus
X. fastidiosa
strains, some specific VNTR loci had a
H
value of 0.83, while the maximum value given by specific RAPD loci was 0.12. Our approach using VNTR markers provides a high-resolution tool for epidemiological, genetic, and ecological analysis of citrus-specific
X. fastidiosa
strains.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
80 articles.
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