Rapid Confirmation of Clostridium perfringens by Using Chromogenic and Fluorogenic Substrates-Reference-Cited by-同舟云学术

Rapid Confirmation of Clostridium perfringens by Using Chromogenic and Fluorogenic Substrates

Published:2001-09 Issue:9 Volume:67 Page:4382-4384
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Adcock Philip W.¹,Saint Christopher P.¹

Affiliation:

1. Microbiology Unit, Australian Water Quality Centre, SA Water Corporation, Salisbury, South Australia 5108, Australia

Abstract

ABSTRACT The use of 4-methylumbelliferyl phosphate (MUP) and ortho -nitrophenyl-β- d -galactopyranoside (ONPG) for the identification of Clostridium perfringens was investigated. A liquid assay containing both MUP and ONPG was a highly specific alternative method for C. perfringens confirmation, reducing incubation time from 48 to only 4 h. The assay solution is easy to prepare, does not require anaerobic conditions for use, and has an extended shelf life.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.67.9.4382-4384.2001

Reference14 articles.

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