Anopheles gambiae Lacking AgTRIO Inefficiently Transmits Plasmodium berghei to Mice-Reference-Cited by-同舟云学术

Anopheles gambiae Lacking AgTRIO Inefficiently Transmits Plasmodium berghei to Mice

Published:2019-09 Issue:9 Volume:87 Page:
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Chuang Yu-Min¹,Freudzon Marianna²,Yang Jing¹,Dong Yuemei³,Dimopoulos George³^ORCID,Fikrig Erol¹⁴

Affiliation:

1. Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, USA

2. Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut, USA

3. Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA

4. Howard Hughes Medical Institute, Chevy Chase, Maryland, USA

Abstract

Antibodies to AgTRIO, a mosquito salivary protein, partially reduce the initial Plasmodium burden in mice. We therefore silenced AgTRIO in mosquitoes and determined the relative contribution of AgTRIO to the ability of Anopheles gambiae to transmit Plasmodium berghei to mice. RNA interference-mediated silencing of AgTRIO in A. gambiae resulted in a 60% reduction in AgTRIO expression. The decrease in AgTRIO expression did not alter the burden of Plasmodium sporozoites in mosquito salivary glands.

Funder

HHS | National Institutes of Health

Howard Hughes Medical Institute

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.00326-19

Reference48 articles.

1. World Health Organization. 2017. World malaria report. World Health Organization, Geneva, Switzerland.

2. Malaria