Affiliation:
1. Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria 3052, Australia
Abstract
ABSTRACT
Replication of the IncL/M plasmid pMU604 is controlled by a small antisense RNA molecule (RNAI), which, by inhibiting the formation of an RNA pseudoknot, regulates translation of the replication initiator protein, RepA. Efficient translation of the
repA
mRNA was shown to require the translation and correct termination of the leader peptide, RepB, and the formation of the pseudoknot. Although the pseudoknot was essential for the expression of
repA
, its presence was shown to interfere with the translation of
repB
. The requirement for pseudoknot formation could in large part be obviated by improving the ribosome binding region of
repA
, either by replacing the GUG start codon by AUG or by increasing the spacing between the start codon and the Shine-Dalgarno sequence (SD). The spacing between the distal pseudoknot sequence and the
repA
SD was shown to be suboptimal for maximal expression of
repA
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
23 articles.
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