Affiliation:
1. Department of Microbiology and Molecular Genetics
2. Center for the Study of Emerging and Reemerging Pathogens, University of Texas Houston Medical School, Houston, Texas 77030
3. Division of Infectious Disease, Department of Medicine
Abstract
ABSTRACT
Eleven PhoP-PhoS homolog pairs were identified by searching the
Enterococcus faecalis
V583 genome sequence database at The Institute for Genomic Research with the
Bacillus subtilis
PhoP-PhoS sequences. Each pair appears to be a potential two-component system composed of a response regulator and a sensor kinase. Seven of the homologs were disrupted in
E. faecalis
strain OG1RF. TX10293, a mutant disrupted in one of these genes (
etaR
, the first gene of the gene pair designated
etaRS
), showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. The predicted EtaR protein sequence showed greatest similarity to LisR of
Listeria monocytogenes
(77%) and CsrR of
Streptococcus pyogenes
(70%); EtaS is 53% similar to LisK and 54% similar to CsrS. When grown in vitro, the TX10293 mutant was more sensitive to low pH (pH 3.4) and more resistant to high temperature (55°C) than wild-type OG1RF. In conclusion, many potential two-component systems are identified for
E. faecalis
, one of which, EtaRS, was shown to be involved in stress response and virulence.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
69 articles.
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