Regiospecific Oxidation of Chlorobenzene to 4-Chlororesorcinol, Chlorohydroquinone, 3-Chlorocatechol and 4-Chlorocatechol by Engineered Toluene o -Xylene Monooxygenases

Author:

Yanık-Yıldırım K. Cansu1,Phul Onkar K.1,Roth Owen S.1,Tlatelpa Areli1,Soria-P Gerardo1,Vardar-Yel Nurcan1,Vardar-Schara Gönül1ORCID

Affiliation:

1. Department of Chemistry, California State University Stanislaus, Turlock, California, USA

Abstract

Chlorobenzene is a commonly used toxic solvent and listed as a priority environmental pollutant by the US Environmental Protection Agency. Here, we report that Escherichia coli TG1 cells expressing toluene o -xylene monooxygenase (ToMO) can successfully oxidize chlorobenzene to form dihydroxy chloroaromatics, which are valuable industrial compounds.

Funder

HHS | National Institutes of Health

CSU | Program for Education and Research in Biotechnology, California State University

U.S. Department of Education

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference50 articles.

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2. Hawley's Condensed Chemical Dictionary, Sixteenth Edition

3. U.S. Environmental Protection Agency. 2009. National Primary Drinking Water Regulations, p 7. US EPA, Washington, DC. Available from https://www.epa.gov/sites/production/files/2016-06/documents/npwdr_complete_table.pdf.

4. Chlorinated Benzenes and Other Nucleus-Chlorinated Aromatic Hydrocarbons

5. Chlorophenols and other related derivatives of environmental concern: Properties, distribution and microbial degradation processes

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