A Simplified Method for Immunological Typing of Trachoma-Inclusion Conjunctivitis-Lymphogranuloma Venereum Organisms-Reference-Cited by-同舟云学术

A Simplified Method for Immunological Typing of Trachoma-Inclusion Conjunctivitis-Lymphogranuloma Venereum Organisms

Published:1973-03 Issue:3 Volume:7 Page:356-360
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Wang San-Pin¹,Kuo Cho-Chou¹,Grayston J. Thomas¹

Affiliation:

1. Department of Pathobiology, School of Public Health and Community Medicine, University of Washington, Seattle, Washington 98195

Abstract

Responding to the pressure of large numbers of trachoma-inclusion conjunctivitis (TRIC)-lymphogranuloma venereum (LGV) isolates from field studies requiring serotyping, we have developed a simplified, less-precise method that utilizes cell culture-grown organisms to produce mouse antisera which is tested agaist prototype TRIC-LGV antigens in the micro-immunofluorescence test. Cell cultures with as few as 5 to 15% of cells showing inclusions produced adequate antibody in mice 4 days after single injection. Knowledge of the reaction of prototype antisera with the antigens has allowed typing of most isolates tested from the pattern of cross-reaction of their antiserum.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.7.3.356-360.1973

Reference11 articles.

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3. Detection of Chlamydia (Bedsonia) in certain infections of man. I. Laboratory procedures: comparison of yolk sac and cell culture for detection and isolation;Gordon F. B.;J. Infect. Dis.,1969

4. Kuo C. C. G. E. Kenny and S. P. Wang. 1971. Trachoma and psittacosis antigens in agar gel double immunodiffusion p. 113-123. In R. L. Nichols (ed.) Trachoma (Proceedings of the Symposium held in Boston Massachusetts August 1970). Excerpta Medica Amsterdam.

5. Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAEdextran;Kuo C. C.;J. Infect. Dis.,1972

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