Affiliation:
1. Institute of Hygiene and Epidemiology, Prague, Czechoslovakia.
Abstract
Nasal and pharyngeal swabs from 134 children with acute respiratory diseases were examined for the presence of respiratory syncytial (RS) virus and adenovirus antigens by enzyme-linked immunosorbent assay (ELISA). The results were compared with those obtained by virus isolation and serology. Altogether, 56 RS virus-positive (prospective study), 51 adenovirus-positive (retrospective study), and 27 negative (prospective study) samples were examined. The sensitivities of ELISA were 96 and 98% for RS virus and adenovirus antigen detection, respectively. No false-positive results were observed. It is concluded that the joint eluate from a nasal and a pharyngeal swab, properly taken and handled, serves well for the detection of RS virus and adenovirus acute respiratory infections by ELISA.
Publisher
American Society for Microbiology
Reference18 articles.
1. Rapid detection of respiratory syncytial virus with a monoclonal antibody;Bell D. M.;J. Clin. Microbiol.,1983
2. Incidence of RS virus infections in premature children's ward;Bruckova M.;J. Hyg. Epidemiol. Microbiol. Immunol.,1979
3. Detection of respiratory syncytial virus in nasal secretions from infants by enzyme-linked immunosorbent assay;Chao R. K.;J. Infect. Dis.,1979
4. Respiratory syncytial virus infection in young hospitalized children: identification of risk patients and prevention of nosocomial spread by rapid diagnosis;Eriksson M.;Acta Paediatr. Scand.,1983
5. Gardner P. S. and J. McQuillin. 1980. Rapid viral diagnosis. 2nd ed. Butterworth & Co. Ltd.. London.
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