Fluorescence Assay for Studying the Ability of Macrolides To Induce Production of Ribosomal Methylase-Reference-Cited by-同舟云学术

Fluorescence Assay for Studying the Ability of Macrolides To Induce Production of Ribosomal Methylase

Published:2002-07 Issue:7 Volume:46 Page:2269-2272
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Clarebout Gervais¹,Leclercq Roland¹

Affiliation:

1. Service de Microbiologie, UPRES EA 2128, Hôpital Côte de Nacre, Université de Caen, 14033 Caen Cedex, France

Abstract

ABSTRACT A screening assay to test the inducing capacity of macrolides by fusing the attenuator of the inducible erm (B) gene from Streptococcus pneumoniae HM28 with the gfpmut1 gene has been designed. Fluorescence was detected under UV light around disks impregnated with inducer macrolides (erythromycin or azithromycin) but not with noninducer ketolides. Induction could be quantified by fluorometry.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.46.7.2269-2272.2002

Reference19 articles.

1. Antibacterial activity of RU 64004 (HMR 3004), a novel ketolide derivative active against respiratory pathogens

2. Bonnefoy, A., A. M Girard, C. Agouridas, and J. F. Chantot. 1997. Ketolides lack inducibility properties of MLS(B) resistance phenotype. J. Antimicrob. Chemother.40:85-90.

3. Ketolides—telithromycin, an example of a new class of antibacterial agents

4. Comité de l'Antibiogramme de la Société Française de Microbiologie. 1996. Technical recommendations for in vitro susceptibility testing. Clin. Microbiol. Infect.2(Suppl. 1):11-25.

5. Denis, A., C. Agouridas, J. M. Auger, Y. Benedetti, A. Bonnefoy, F. Bretin, J. F. Chantot, A. Dussarat, C. Fromentin, S. G. D'Ambrieres, S. Lachaud, P. Laurin, O. Le Martret, V. Loyau, N. Tessot, J. M. Pejac, and S. Perron. 1999. Synthesis and antibacterial activity of HMR 3647 a new ketolide highly potent against erythromycin-resistant and susceptible pathogens. Bioorg. Med. Chem. Lett.9:3075-3080.

Cited by 16 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Streptococcus pyogenes infects human endometrium by limiting the innate immune response;J CLIN INVEST;2021

2. Streptococcuspyogenes infects human endometrium by limiting the innate immune response;Journal of Clinical Investigation;2021-02-15

3. Floresans ışımaya bağlı minimal inhibisyon konsantrasyon (MİK) belirlenmesi;Harran Üniversitesi Veteriner Fakültesi Dergisi;2020-06-25

4. Streptococcus pyogenes infects human endometrium by limiting its immune response;2019-07-25

5. Inducible expression of erm(B) by the ketolides telithromycin and cethromycin;International Journal of Antimicrobial Agents;2015-08