Fatty Acid Desaturase Mutants of Saccharomyces cerevisiae

Author:

Keith A. D.12,Resnick M. R.12,Haley A. B.12

Affiliation:

1. Department of Genetics, University of California, Berkeley, California 94720

2. Department of Medical Physics, University of California, Berkeley, California 94720

Abstract

Genetic and biochemical analyses were conducted on fatty acid mutants of yeast deficient for Δ 9 -desaturase activity in the production of palmitoleate and oleate. Two genetic loci were observed and two others are inferred; three of these were represented by respiratory-deficient (petite) strains. All strains were incapable of converting palmitate to palmitoleate and stearate to oleate whether the direct precursor or acetate was followed. All strains were capable of acylating both de novoproduced fatty acids and oleate taken up from the medium into phospholipids and neutral lipids. Two revertants were analyzed which differed in their ability to produce palmitoleate and oleate.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference22 articles.

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3. Methods for the analysis of lipids extracted from human arteries and other tissues;Bottcher C. J. F.;Rec. Trav. Chim. Pays-Bas,1959

4. Biotin and bacterial growth. I. Relation to aspartate, oleate, and carbon dioxide;Broquist H. P.;J. Biol. Chem.,1951

5. Role of fatty acids in the growth stimulation of Sarcina species by vitamin-free casein digests;Demain A. L.;J. Bacteriol.,1959

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