Effect of aerobic and anaerobic atmospheres on isolation of organisms from blood cultures

Author:

Blazevic D J,Stemper J E,Matsen J M

Abstract

Blood was cultured in two vacuum bottles containing Columbia broth. Filtered air was admitted to one bottle (aerobic); the unvented bottle was considered anaerobic. Cultures were incubated at 35 C until growth occurred or for at least 7 days. Of 744 organisms isolated, 50% were isolated from both bottles, 30% from the aerobic bottle only, and 20% from the anaerobic bottle only. These results indicate the need for use of both an aerobic and anaerobic bottle for blood cultures.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference5 articles.

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1. Biographical Feature: John Matsen, M.D.;Journal of Clinical Microbiology;2014-06-11

2. Effect of Aerobic and Anaerobic Atmosphere on the Detection of Micro-Organisms From Blood Cultures;Acta Pathologica Microbiologica Scandinavica Series B: Microbiology;2009-08-15

3. Utility of Anaerobic Blood Cultures for the Isolation of Nonanaerobic Organisms;Infectious Diseases in Clinical Practice;2002-08

4. Update on detection of bacteremia and fungemia;Clinical Microbiology Reviews;1997-07

5. Laboratory Detection of Anaerobic Bacteremia;Clinics in Laboratory Medicine;1994-03

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