Evaluation of Cytomegalovirus (CMV) DNA Quantification in Dried Blood Spots: Retrospective Study of CMV Congenital Infection-Reference-Cited by-同舟云学术

Evaluation of Cytomegalovirus (CMV) DNA Quantification in Dried Blood Spots: Retrospective Study of CMV Congenital Infection

Published:2007-11 Issue:11 Volume:45 Page:3804-3806
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Vauloup-Fellous Christelle¹,Ducroux Aurélie²,Couloigner Vincent³,Marlin Sandrine⁴,Picone Olivier⁵,Galimand Julie²,Loundon Natalie⁴,Denoyelle Françoise⁴,Grangeot-Keros Liliane¹,Leruez-Ville Marianne²

Affiliation:

1. Service de Microbiologie Immunologie Biologique, Assistance Publique-Hôpitaux de Paris, Hôpital Antoine Béclère, Université Paris Sud 11, 157 Avenue de la Porte de Trivaux, 92141 Clamart, France

2. Laboratoire de Virologie, Hôpital Necker-Enfants-Malades, Assistance Publique-Hôpitaux de Paris, Université Paris Descartes, EA 36-20, 149 rue de Sèvres, 75015 Paris, France

3. Service d'ORL Pédiatrique CHU Necker-Enfants-Malades, Assistance Publique-Hôpitaux de Paris, Université Paris Descartes, 149 rue de Sèvres, 75015 Paris, France

4. Service d'ORL Hôpital Trousseau, Assistance Publique-Hôpitaux de Paris, 26 Avenue du Dr. Arnold Netter, 75571 Paris, Cedex 12, France

5. Service de Gynécologie-Obstétrique, Assistance Publique- Hôpitaux de Paris, Hôpital Antoine Béclère, Université Paris Sud 11, 157 Avenue de la Porte de Trivaux, 92141 Clamart, France

Abstract

ABSTRACT We compared two protocols for extracting DNA from dried blood spots for cytomegalovirus (CMV) DNA detection and quantification by real-time PCR. Both extraction methods were reliable for the retrospective diagnosis of CMV congenital infection. Quantification of CMV DNA was valuable after normalization of viral loads with albumin gene PCR amplification results.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01654-07

Reference17 articles.

1. Barbi, M., S. Binda, S. Caroppo, A. Calvario, C. Germinario, A. Bozzi, M. L. Tanzi, L. Veronesi, I. Mura, A. Piana, G. Solinas, L. Pugni, G. Bevilaqua, and F. Mosca. 2006. Multicity Italian study of congenital cytomegalovirus infection. Pediatr. Infect. Dis. J.25:156-159.

2. Barbi, M., S. Binda, V. Primache, S. Caroppo, P. Dido, P. Guidotti, C. Corbetta, and D. Melotti. 2000. Cytomegalovirus DNA detection in Guthrie cards: a powerful tool for diagnosing congenital infection. J. Clin. Virol.17:159-165.

3. Binda, S., S. Caroppo, P. Dido, V. Primache, L. Veronesi, A. Calvario, A. Piana, and M. Barbi. 2004. Modification of CMV DNA detection from dried blood spots for diagnosing congenital CMV infection. J. Clin. Virol.30:276-279.

4. Boppana, S. B., K. B. Fowler, R. F. Pass, L. B. Rivera, R. D. Bradford, F. D. Lakeman, and W. J. Britt. 2005. Congenital cytomegalovirus infection: association between virus burden in infancy and hearing loss. J. Pediatr.146:817-823.

5. Distefano, A. L., A. Alonso, F. Martin, and F. Pardon. 2004. Human cytomegalovirus: detection of congenital and perinatal infection in Argentina. BMC Pediatr.4:11.

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