Author:
Cohen J I,Ticehurst J R,Feinstone S M,Rosenblum B,Purcell R H
Abstract
A full-length cDNA copy of an attenuated, cell culture-adapted hepatitis A virus (HAV HM-175/7 MK-5) genome was constructed in the PstI site of plasmid vector pBR322. Transfection of monkey kidney cells with this plasmid failed to induce the production of hepatitis A virus (HAV). The HAV cDNA was excised from pBR322 and inserted, without the oligo(dG) X oligo(dC) tails, into an RNA transcription vector to yield plasmid pHAV/7. Transfection of monkey kidney cells with pHAV/7 DNA induced HAV infection. Transfection with RNA transcripts produced in vitro from pHAV/7 yielded about 10-fold more HAV than did transfection with pHAV/7 DNA. Marmosets inoculated with transfection-derived virus developed anti-HAV antibodies and had liver enzyme patterns that closely resembled the liver enzyme patterns seen in animals inoculated with virus from a comparable level of cell culture passage. Infectious RNA transcripts from HAV cDNA should be useful for studying the molecular basis of cell culture adaptation and attenuation as well as for studying specific viral functions.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference26 articles.
1. Almond J. W. G. D. Westrop A. J. Cann G. Stanway D. M. A. Evans P. D. Minor and G. C. Schild. 1985. Attenuation and reversion to neurovirulence of the Sabin poliovirus type-3 vaccine p. 271-277. In R. A. Lerner R. M. Chanock and F. Brown (ed.) Vaccines 85: molecular and chemical basis of resistance to parasitic bacterial and viral diseases. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
2. Genetic complementation among poliovirus mutants derived from an infectious cDNA clone;Bernstein H. D.;J. Virol.,1986
3. Poliovirus mutant that does not selectively inhibit host cell protein synthesis;Bernstein H. D.;Mol. Cell. Biol.,1985
4. Complete nucleotide sequence of an attenuated hepatitis A virus: comparison with wild-type virus;Cohen J. I.;Proc. Natl. Acad. Sci. USA,1987
5. Complete nucleotide sequence of wild-type hepatitis A virus: comparison with different strains of hepatitis A virus and other picornaviruses;Cohen J. I.;J. Virol.,1987
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