Simultaneous Detection of Staphylococcus aureus and Coagulase-Negative Staphylococci in Positive Blood Cultures by Real-Time PCR with Two Fluorescence Resonance Energy Transfer Probe Sets-Reference-Cited by-同舟云学术

Simultaneous Detection of Staphylococcus aureus and Coagulase-Negative Staphylococci in Positive Blood Cultures by Real-Time PCR with Two Fluorescence Resonance Energy Transfer Probe Sets

Published:2004-12 Issue:12 Volume:42 Page:5739-5744
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Sakai H.¹,Procop G. W.²,Kobayashi N.¹,Togawa D.¹,Wilson D. A.²,Borden L.³,Krebs V.³,Bauer T. W.¹

Affiliation:

1. Department of Anatomic Pathology, Section of Surgical Pathology

2. Department of Clinical Pathology, Section of Clinical Microbiology

3. Department of Orthopaedic Surgery, The Cleveland Clinic Foundation, Cleveland, Ohio

Abstract

ABSTRACT A real-time PCR assay that uses two fluorescence resonance energy transfer probe sets and targets the tuf gene of staphylococci is described here. One probe set detects the Staphylococcus genus, whereas the other probe set is specific for Staphylococcus aureus . One hundred thirty-eight cultured isolates, which contained 41 isolates of staphylococci representing at least nine species, and 100 positive blood cultures that contained gram-positive cocci in clusters were tested. This assay was 100% sensitive and 100% specific for the detection of the Staphylococcus genus and of S. aureus .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.12.5739-5744.2004

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