Spoligotyping Profile Change Caused by Deletion of a Direct Variable Repeat in a Mycobacterium tuberculosis Isogenic Laboratory Strain-Reference-Cited by-同舟云学术

Spoligotyping Profile Change Caused by Deletion of a Direct Variable Repeat in a Mycobacterium tuberculosis Isogenic Laboratory Strain

Published:2004-11 Issue:11 Volume:42 Page:5388-5391
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Aranaz Alicia¹,Romero Beatriz¹,Montero Natalia¹,Álvarez Julio¹,Bezos Javier¹,de Juan Lucía¹,Mateos Ana¹,Domínguez Lucas¹

Affiliation:

1. Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain

Abstract

ABSTRACT Spoligotyping is a major tool for molecular typing of Mycobacterium tuberculosis complex organisms. For epidemiological purposes, strains are considered clonal only when their spoligotyping patterns are identical. We report a change in the spoligotyping profiles of truly isogenic strains (a clinical isolate and a subculture derived in the laboratory) caused by deletion of a direct variable repeat. Without the information about the relationship between them, a link between these strains would have gone unnoticed. Evolutionary events should be taken into account in the interpretation of spoligotyping results and in the design of databases.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.11.5388-5391.2004

Reference35 articles.

1. GenotypicAnalysis of Mycobacterium tuberculosis in Bangladesh andPrevalence of the BeijingStrain

2. Usefulness of Spoligotyping To Discriminate IS 6110 Low-Copy-Number Mycobacterium tuberculosis Complex Strains Cultured in Denmark

3. Bifani, P. J., B. Mathema, Z. Liu, S. L. Moghazeh, B Shopsin, B. Tempalski, J. Driscol, R. Frothingham, J. M. Muser, P. Alcabes, and B. N. Kreiswirth. 1999. Identification of a W variant outbreak of Mycobacterium tuberculosis via population-based molecular epidemiology. JAMA282:2321-2327.

4. Caminero, J. A., M. J. Pena, M. I. Campos-Herrero, J. C. Rodríguez, I. García, P. Cabrera, C. Lafoz, S. Samper, H. Takiff, O. Afonso, J. M. Pavón, M. J. Torres, D. van Soolingen, D. A. Enarson, and C. Martín. 2001. Epidemiological evidence of the Beijing genotype on Gran Canaria island. Am. J. Respir. Crit. Care Med.164:1165-1170.

5. Corner, L. A., and A. C. Trajstman. 1988. An evaluation of 1-hexadecyl-pyridinium chloride as a decontaminant in the primary isolation of Mycobacterium bovis from bovine lesions. Vet. Microbiol.18:127-134.

Cited by 15 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The effect of in vitro consecutive passages and culture medium on the genetic variations in BCG Pasteur 1173P2 vaccine;PLOS ONE;2023-01-23

2. Mini-review on CRISPR-Cas9 and its potential applications to help controlling neglected tropical diseases caused by Trypanosomatidae;Infection, Genetics and Evolution;2018-09

3. Genetic diversity assessment of Tunisian Mycobacterium bovis population isolated from cattle;BMC Veterinary Research;2017-12

4. Mathematical Models for the Epidemiology and Evolution of Mycobacterium tuberculosis;Advances in Experimental Medicine and Biology;2017

5. Molecular typing of drug-susceptible and -resistant Mycobacterium tuberculosis in Johannesburg, South Africa;The International Journal of Tuberculosis and Lung Disease;2015-07-01