Comparison of PCR for Sputum Samples Obtained by Induced Cough and Serological Tests for Diagnosis of Mycoplasma pneumoniae Infection in Children-Reference-Cited by-同舟云学术

Comparison of PCR for Sputum Samples Obtained by Induced Cough and Serological Tests for Diagnosis of Mycoplasma pneumoniae Infection in Children

Published:2006-06 Issue:6 Volume:13 Page:708-710
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Yamazaki Tsutomu¹²³,Narita Mitsuo¹²³,Sasaki Nozomu¹²³,Kenri Tsuyoshi¹²³,Arakawa Yoshichika¹²³,Sasaki Tsuguo¹²³

Affiliation:

1. Department of Pediatrics, Saitama Medical School, Saitama

2. Department of Pediatrics, Sapporo Tetsudo (JR) Hospital, Hokkaido

3. Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan

Abstract

ABSTRACT Passive agglutination (PA) and immunoglobulin M (IgM), IgA, and IgG enzyme-linked immunosorbent assays (ELISAs) for the diagnosis of Mycoplasma pneumoniae were compared with PCR testing of sputum samples obtained from children with lower respiratory tract infections. The sensitivity and specificity of PA were 80.3% and 92.3% at a titer of 1:80. ELISA was found to be less sensitive than PA.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CVI.00413-05

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