Monocyte CD163 and CD36 Expression in Human Whole Blood and Isolated Mononuclear Cell Samples: Influence of Different Anticoagulants

Author:

Moniuszko Marcin12,Kowal Krzysztof12,Rusak Malgorzata12,Pietruczuk Miroslawa12,Dabrowska Milena12,Bodzenta-Lukaszyk Anna12

Affiliation:

1. Department of Allergology and Internal Medicine, Medical University of Bialystok, 15-276 Bialystok, Poland

2. Department of Hematological Diagnostics, Medical University of Bialystok, 15-274 Bialystok, Poland

Abstract

ABSTRACT We investigated whether the choice of anticoagulant or the application of density gradient mononuclear cell isolation may account for conflicting published data regarding the levels of the scavenger receptors' expression in healthy individuals. We demonstrate that the detection of CD163, but not CD36, differs dramatically among the methods.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

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