Affiliation:
1. Becton, Dickinson and Co. and Baltimore Biological Laboratory, Baltimore, Maryland
Abstract
An anaerobic system is described which allows the microbiologist or hospital technician ease of operation not previously possible with other systems. Gas cylinders, vacuum pumps, valves, and gauges have been eliminated. A new anaerobic lid was developed that is fitted only with a snap-in rubber gasket, a double stainless-steel gauze flash arrestor, and a catalyst holder. The holder contains a palladium pellet catalyst which is active at room temperature and requires no heat for activation. This system was made specifically for use with a disposable hydrogen-carbon dioxide generator and a disposable methylene blue anaerobic indicator. In addition to ease of operation, this jar incorporates unique safety features which eliminate the possibility of a laboratory explosion. An oxygen-free atmosphere composed primarily of nitrogen and carbon dioxide was quickly achieved within the jar to insure maximal growth.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference11 articles.
1. A modification of the Brown anaerobe jar;BREWER J. H.;J. Lab. Clin. Med.,1939
2. Disposable hydrogen generator;BREWER J. H.;Science,1965
3. Improved anaerobic indicator;BREWER J. H.;Appl. Microbiol.,1966
4. The use of sodium borohydride for producing hydrogen in an anaerobe jar;BREWER J. H.;Appl. Microbiol.,1955
5. An improved anaerobe jar;BROWN J. H.;J. Exptl. Med.,1921
Cited by
22 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献