Replication of Coxsackievirus B3 in Primary Cell Cultures Generates Novel Viral Genome Deletions-Reference-Cited by-同舟云学术

Replication of Coxsackievirus B3 in Primary Cell Cultures Generates Novel Viral Genome Deletions

Published:2008-02-15 Issue:4 Volume:82 Page:2033-2037
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Kim K.-S.¹,Chapman N. M.¹,Tracy S.¹

Affiliation:

1. Enterovirus Research Laboratory, Department of Pathology and Microbiology, University of Nebraska Medical Center, 986495 Nebraska Medical Center, Omaha, Nebraska 68198-6495

Abstract

ABSTRACT Coxsackievirus B3 (CVB3) generates 5′-terminally deleted genomes (TDs) during replication in murine hearts. We show here that CVB3 populations with TDs can also be generated within two to three passages of CVB3 in primary, but not immortalized, cell cultures. Deletions of less than 49 nucleotides increase in size during passage, while 5′ TDs of 49 nucleotides appear to be the maximum deletion size. The cellular environment of contact-inhibited primary cell cultures or the myocardium in vivo is sufficient for the selection of 5′ TDs over undeleted genomes.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01774-07

Reference25 articles.

1. Andino, R., G. E. Rieckhof, and D. Baltimore. 1990. A functional ribonucleoprotein complex forms around the 5′ end of poliovirus RNA. Cell63:369-380.

2. Andreoletti, L., T. Bourlet, D. Moukassa, L. Rey, D. Hot, Y. Li, V. Lambert, B. Gosselin, J. F. Mosnier, C. Stankowiak, and P. Wattre. 2000. Enteroviruses can persist with or without active viral replication in cardiac tissue of patients with end-stage ischemic or dilated cardiomyopathy. J. Infect. Dis.182:1222-1227.

3. Baboonian, C., M. J. Davies, J. Booth, and W. McKenna. 1997. Coxsackie B viruses and heart disease. Curr. Top. Microbiol. Immunol.223:31-52.

4. Bedard, K. M., and B. L. Semler. 2004. Regulation of picornavirus gene expression. Microbes Infect.6:702-713.

5. Functional Interaction of Heterogeneous Nuclear Ribonucleoprotein C with Poliovirus RNA Synthesis Initiation Complexes

Cited by 48 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Enterovirus and parechovirus infections;Remington and Klein's Infectious Diseases of the Fetus and Newborn Infant;2025

2. Major Group-B Enterovirus populations deleted in the noncoding 5’ region of genomic RNA modulate activation of the type I interferon pathway in cardiomyocytes and induce myocarditis;PLOS Pathogens;2024-05-02

3. Enterovirus-Cardiomyocyte Interactions: Impact of Terminally Deleted Genomic RNAs on Viral and Host Functions;Journal of Virology;2023-01-31

4. Enterovirus-cardiomyocyte interactions: impact of terminally deleted genomic RNAs on viral and host functions;2022-09-16

5. Early Emergence of 5′ Terminally Deleted Coxsackievirus-B3 RNA Forms Is Associated with Acute and Persistent Infections in Mouse Target Tissues;Vaccines;2022-07-28