Shipment Impairs Lymphocyte Proliferative Responses to Microbial Antigens

Author:

Betensky Rebecca A.1,Connick Elizabeth2,Devers Jennifer3,Landay Alan L.4,Nokta Mostafa5,Plaeger Susan6,Rosenblatt Howard7,Schmitz John L.8,Valentine Fred9,Wara Diane10,Weinberg Adriana2,M. Lederman Howard3

Affiliation:

1. Harvard School of Public Health, Boston, Massachusetts1;

2. University of Colorado Health Sciences Center, Denver, Colorado2;

3. Johns Hopkins University School of Medicine, Baltimore, Maryland3;

4. Rush Medical College, Chicago, Illinois4;

5. University of Texas Medical Branch, Galveston,5 and

6. UCLA School of Medicine, Los Angeles,6 and

7. Baylor College of Medicine, Houston,7Texas;

8. University of North Carolina, Chapel Hill, North Carolina8; and

9. New York University Medical Center, New York, New York9

10. UCSF School of Medicine, San Francisco,10 California;

Abstract

ABSTRACT Lymphocyte proliferation assays (LPAs) are widely used to assess T-lymphocyte function of patients with human immunodeficiency virus infection and other primary and secondary immunodeficiency disorders. Since these assays require expertise not readily available at all clinical sites, specimens may be shipped to central labs for testing. We conducted a large multicenter study to evaluate the effects of shipping on assay performance and found significant loss of LPA activity. This may lead to erroneous results for individual subjects and introduce bias into multicenter trials.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Reference12 articles.

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2. In vitro T cell function, delayed-type hypersensitivity skin testing, and CD4+ T cell subset phenotyping independently predict survival time in patients infected with human immunodeficiency virus.;Dolan M. J.;J. Infect. Dis.,1995

3. Use of whole blood methods in assessment of immune parameters in immunodeficiency states.;Fletcher M. A.;Diagn. Clin. Immunol.,1987

4. Fletcher M. A. Urban R. G. Asthana D. Walling J. Friedlander A. Page J. B. Lymphocyte proliferation Manual of clinical laboratory immunology 5th ed. Rose N. R. Conway de Macarco E. Folds J. D. Lane H. C. Nakamura R. M. 1997 313 319 ASM Press Washington D.C.

5. Gern J. E. Lederman H. M. Immunodeficiency Laboratory medicine. The selection and interpretation of clinical laboratory studies. Noe D. A. Rock R. C. 1994 254 275 Williams and Wilkins Baltimore Md

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