Rapid Detection of Mutations in the 23S rRNA Gene of Helicobacter pylori That Confers Resistance to Clarithromycin Treatment to the Bacterium-Reference-Cited by-同舟云学术

Rapid Detection of Mutations in the 23S rRNA Gene of Helicobacter pylori That Confers Resistance to Clarithromycin Treatment to the Bacterium

Published:2001-02 Issue:2 Volume:39 Page:691-695
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Matsumura Masayuki¹,Hikiba Yoko¹,Ogura Keiji¹²,Togo Goichi²,Tsukuda Izumi¹,Ushikawa Kenji¹,Shiratori Yasushi²,Omata Masao²

Affiliation:

1. The Institute for Adult Diseases, Asahi Life Foundation, 1-9-14 Nishi-Shinjuku, Shinjuku-ku, Tokyo 160-0023,1 and

2. Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655,2 Japan

Abstract

ABSTRACT We developed a new method capable of detecting point mutations in the 23S rRNA gene of Helicobacter pylori using a LightCycler. Our method can detect a mutation in this gene in less than 1 h and can process many samples at once, thereby contributing to the selection of patients suitable for clarithromycin-based therapy.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.2.691-695.2001

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