The Nucleoprotein Is Required for Efficient Coronavirus Genome Replication-Reference-Cited by-同舟云学术

The Nucleoprotein Is Required for Efficient Coronavirus Genome Replication

Published:2004-11-15 Issue:22 Volume:78 Page:12683-12688
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Almazán Fernando¹,Galán Carmen¹,Enjuanes Luis¹

Affiliation:

1. Department of Molecular and Cell Biology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Cientificas, Campus Universidad Autónoma, Cantoblanco, Madrid, Spain

Abstract

ABSTRACT The construction of a set of transmissible gastroenteritis coronavirus (TGEV)-derived replicons as bacterial artificial chromosomes is reported. These replicons were generated by sequential deletion of nonessential genes for virus replication, using a modified TGEV full-length cDNA clone containing unique restriction sites between each pair of consecutive genes. Efficient activity of TGEV replicons was associated with the presence of the nucleoprotein provided either in cis or in trans . TGEV replicons were functional in several cell lines, including the human cell line 293T, in which no or very low cytopathic effect was observed, and expressed high amounts of heterologous protein.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.78.22.12683-12688.2004

Reference39 articles.

1. Agapov, E. V., I. Frolov, B. D. Lindenbach, B. M. Pragai, S. Schlesinger, and C. M. Rice. 1998. Noncytopathic Sindbis virus RNA vectors for heterologous gene expression. Proc. Natl. Acad. Sci. USA95:12989-12994.

2. Almazán, F., J. M. González, Z. Pénzes, A. Izeta, E. Calvo, J. Plana-Durán, and L. Enjuanes. 2000. Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome. Proc. Natl. Acad. Sci. USA97:5516-5521.

3. Transcription Regulatory Sequences and mRNA Expression Levels in the Coronavirus Transmissible Gastroenteritis Virus

4. Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus

5. Delmas, B., J. Gelfi, H. Sjöström, O. Noren, and H. Laude. 1994. Further characterization of aminopeptidase-N as a receptor for coronaviruses. Adv. Exp. Med. Biol.342:293-298.

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