Affiliation:
1. Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164
2. Department of Animal Sciences, University of Illinois, Urbana-Champaign, Illinois 61801
Abstract
ABSTRACT
Cattle infected with
Neospora caninum
readily experience transplacental parasite transmission, presumably after maternal parasitemia, leading to abortion or birth of congenitally infected calves. Cytotoxic T lymphocytes (CTL) are important mediators of protective immunity against
Toxoplasma gondii
, an intracellular apicomplexan protozoan closely related to
N. caninum
. In this study,
N. caninum-
specific CTL expanded from peripheral blood mononuclear cells of two major histocompatibility complex-mismatched, experimentally infected cattle were identified by using a
51
Cr release cytotoxicity assay. Enrichment and blocking of CD4
+
- and CD8
+
-T-lymphocyte effector subsets indicated that CD4
+
CTL killed
N. caninum
-infected, autologous target cells and that killing was mediated through a perforin/granzyme pathway. Detection and characterization of CTL responses to
N. caninum
in the natural, outbred, bovine host will facilitate identification of immunogens and design of immunization strategies to induce parasite-specific CTL against transplacental
N. caninum
transmission in cattle.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference53 articles.
1. Anderson, M. L., C. W. Palmer, M. C. Thurmond, J. P. Picanso, P. C. Blanchard, R. E. Breitmeyer, A. W. Layton, M. McAllister, B. Daft, H. Kinde, et al. 1995. Evaluation of abortions in cattle attributable to neosporosis in selected dairy herds in California. J. Am. Vet. Med. Assoc.207:1206-1210.
2. Anderson, M. L., J. P. Reynolds, J. D. Rowe, K. W. Sverlow, A. E. Packham, B. C. Barr, and P. A. Conrad. 1997. Evidence of vertical transmission of Neospora sp. infection in dairy cattle. J. Am. Vet. Med. Assoc.210:1169-1172.
3. Andersson, L., and L. Rask. 1988. Characterization of the MHC class II region in cattle: the number of DQ genes varies between haplotypes. Immunogenetics27:110-120.
4. Appay, V., J. J. Zaunders, L. Papagno, J. Sutton, A. Jaramillo, A. Waters, P. Easterbrook, P. Grey, D. Smith, A. J. McMichael, D. A. Cooper, S. L. Rowland-Jones, and A. D. Kelleher. 2002. Characterization of CD4+ CTLs ex vivo. J. Immunol.168:5954-5958.
5. Validation of a Commercially Available Monoclonal Antibody-Based Competitive-Inhibition Enzyme-Linked Immunosorbent Assay for Detection of Serum Antibodies to
Neospora caninum
in Cattle
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