Affiliation:
1. Division of Microbiology and Infectious Diseases, Western Australian Centre for Pathology and Medical Research, Nedlands, Australia
Abstract
ABSTRACT
Isolation of
Burkholderia pseudomallei
currently relies on the use of Ashdown's selective agar (ASA). We designed a new selective agar (
Burkholderia pseudomallei
selective agar [BPSA]) to improve recovery of the more easily inhibited strains of
B. pseudomallei. B. pseudomallei
,
Burkholderia cepacia
, and
Pseudomonas aeruginosa
were used to determine the selectivity and sensitivity of BPSA. BPSA was more inhibitory to
P. aeruginosa
and
B. cepacia
and should make recognition of
Burkholderia
species easier due to distinctive colony morphology. BPSA also inhibited
Enterococcus
,
Escherichia
,
Staphylococcus
, and
Streptococcus
. These results indicate that BPSA is a potential replacement for ASA.
Publisher
American Society for Microbiology
Reference8 articles.
1. Ashdown, L. R. 1979. An improved screening technique for isolation of Pseudomonas pseudomallei from clinical specimens. Pathology11:293-297.
2. Brook, M. D., B. Currie, and P. M. Desmarchelier. 1997. Isolation and identification of Burkholderia pseudomallei from soil using selective culture techniques and the polymerase chain reaction. J. Appl. Microbiol.82:589-596.
3. Inglis, T. J. J., S. C. Garrow, C. Adams, M. Henderson, and M. Mayo. 1998. Dry-season outbreak of melioidosis in Western Australia. Lancet352:1600.
4. Inglis, T. J. J., S. C. Garrow, C. Adams, M. Henderson, M. Mayo, and B. J. Currie. 1999. Acute melioidosis outbreak in Western Australia. Epidemiol. Infect.123:437-443.
5. Inglis, T. J. J., B. J. Mee, and B. J. Chang. 2000. The environmental microbiology of melioidosis. Rev. Med. Microbiol.12:13-20.
Cited by
52 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献