Radiometric method for testing susceptibility of mycobacteria to pyrazinamide in 7H12 broth

Author:

Heifets L B,Iseman M D

Abstract

The test of susceptibility to pyrazinamide requires an acid environment (pH less than or equal to 5.5). This, however, is not favorable to the growth of Mycobacterium tuberculosis, especially in solid agar media. To obviate this difficulty, we developed a testing method with 7H12 broth medium and based on radiometric readings of the growth. The radiometric method employed in this study (BACTEC system) provides an opportunity to detect the dynamics of growth by daily recording of the growth index, which reflects the metabolic activity of the multiplying bacteria. In our technique, M. tuberculosis isolates were initially cultivated at pH 6.8. After logarithmic growth had begun, phosphoric acid solution was added to obtain pH 5.5. When pyrazinamide was added simultaneously with the acid, the growth index of susceptible cultures decreased, whereas it continued to increase in pH 5.5 control vials and in tests with pyrazinamide-resistant strains.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference15 articles.

1. A simple way of detecting pyrazinamide resistance;Bander E.;Tubercle,1972

2. Improved method for testing susceptibility of Mycobacterium tuberculosis to pyrazinamide;Butler W. R.;J. Clin. Microbiol.,1982

3. Susceptibility of Mycobacterium tuberculosis to pyrazinamide and its relationship to pyrazinamidase activity;Butler W. R.;Antimicrob. Agents Chemother.,1983

4. Radiometric detection of the metabolic activity of Mycobacterium tuberculosis;Cummings D. M.;J. Nucl. Med.,1975

5. Pyrazinamide susceptibility and amidase activity of tubercle bacilli;Konno K.;Am. Rev. Respir. Dis.,1967

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3