Sandwich enzyme immunoassay and latex agglutination test for herpes simplex virus keratitis

Author:

Lee S F1,Pepose J S1

Affiliation:

1. Department of Ophthalmology, Washington University School of Medicine, St. Louis, Missouri 63110.

Abstract

We determined the in vitro sensitivities and the diagnostic efficacies of a commercially available latex agglutination test and an enzyme-linked immunosorbent assay using a rabbit model of herpes simplex virus type 1 dendritic keratitis. In contrast to the latex agglutination test, the HERPCHEK enzyme-linked immunosorbent assay offers an extremely sensitive and reliable means of laboratory diagnosis of experimental herpes simplex virus keratitis and is more rapid than viral isolation in tissue culture.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference8 articles.

1. Comparison of ELISA with virus isolation for the diagnosis of genital herpes;Alexander I.;J. Clin. Pathol.,1985

2. Clinical evaluation of a new herpes simplex virus ELISA: a rapid diagnostic test for herpes simplex virus;Baker D. A.;Obstet. Gynecol.,1989

3. Rapid detection of herpes simplex virus (HSV) antigen in human ocular infections;Dunkel E. C.;Curr. Eye Res.,1988

4. Detection of herpes simplex virus in clinical specimens by an enzyme-linked immunosorbent assay;Lawrence T. G.;Am. J. Clin. Pathol.,1984

5. Application of immunologic technology to the diagnosis of viral infections of the ocular surface;Pepose J. S.;Cornea,1988

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