Affiliation:
1. Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada
Abstract
ABSTRACT
Adult
Drosophila melanogaster
raised in the absence of symbiotic bacteria have fewer intestinal stem cell divisions and a longer life span than their conventionally reared counterparts. However, we do not know if increased stem cell divisions are essential for symbiont-dependent regulation of longevity. To determine if individual symbionts cause aging-dependent death in
Drosophila
, we examined the impacts of common symbionts on host longevity. We found that monoassociation of adult
Drosophila
with
Lactobacillus plantarum
, a widely reported fly symbiont and member of the probiotic
Lactobacillus
genus, curtails adult longevity relative to germfree counterparts. The effects of
Lactobacillus plantarum
on life span were independent of intestinal aging. Instead, we found that association with
Lactobacillus plantarum
causes an extensive intestinal pathology within the host, characterized by loss of stem cells, impaired epithelial renewal, and a gradual erosion of epithelial ultrastructure. Our study uncovers an unknown aspect of
Lactobacillus plantarum
-
Drosophila
interactions and establishes a simple model to characterize symbiont-dependent disruption of intestinal homeostasis.
IMPORTANCE
Under homeostatic conditions, gut bacteria provide molecular signals that support the organization and function of the host intestine. Sudden shifts in the composition or distribution of gut bacterial communities impact host receipt of bacterial cues and disrupt tightly regulated homeostatic networks. We used the
Drosophila melanogaster
model to determine the effects of prominent fly symbionts on host longevity and intestinal homeostasis. We found that monoassociation with
Lactobacillus plantarum
leads to a loss of intestinal progenitor cells, impaired epithelial renewal, and disruption of gut architecture as flies age. These observations uncover a novel phenotype caused by monoassociation of a germfree host with a common symbiont and establish a simple model to characterize symbiont-dependent loss of intestinal homeostasis.
Funder
Canadian Institutes of Health Research
Publisher
American Society for Microbiology
Cited by
37 articles.
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