Urine sample used for congenital toxoplasmosis diagnosis by PCR

Author:

Fuentes I1,Rodriguez M1,Domingo C J1,del Castillo F1,Juncosa T1,Alvar J1

Affiliation:

1. Servicio de Parasitología, Instituto de Salud Carlos III, Madrid, Spain.

Abstract

The diagnosis of toxoplasmosis in congenitally infected infants can be difficult; serology is unreliable, and diagnosis must be based on the combination of symptomatology and direct demonstration of the parasite. Four infants suspected of having Toxoplasma gondii infection were studied by serological analysis, tissue culture, and PCR determination. T. gondii was isolated from the urine of one patient. The parasite was detected by PCR in the blood and cerebrospinal fluid of three infants and in the urine in all patients. Because nested PCR proved to be a sensitive, relatively rapid, and specific method and because it can be applied to a variety of different clinical samples, PCR can be a valuable technique for the identification of T. gondii infections in children. The present study indicates that PCR examination of urine, a fluid never before used for diagnosis in this age group, may be valuable in diagnosing cases of congenital toxoplasmosis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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5. Cristina N. F. Derouin H. Pelloux R. Pierce M. F. Cesbron-Delauwn and P. Ambroise-Thomas. 1992. Detection de Toxoplasma gondii chez des patients sidéens par la technique de "polymerase chain reaction" (PCR) à l'aide de la sequence répétée TGR1E. Pathol. Biol. 40:52-55.

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