Expanded Multilocus Sequence Typing for Burkholderia Species

Author:

Spilker Theodore1,Baldwin Adam2,Bumford Amy1,Dowson Chris G.2,Mahenthiralingam Eshwar3,LiPuma John J.1

Affiliation:

1. Division of Infectious Diseases, Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan

2. Department of Biological Sciences, Warwick University, Coventry, United Kingdom

3. Cardiff School of Biosciences, Cardiff University, Cardiff, United Kingdom

Abstract

ABSTRACT PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B . cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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