Affiliation:
1. Center for Cell Signaling, University of Virginia, Charlottesville, Virginia 22908
Abstract
ABSTRACT
Chromatin remodeling is central to the regulation of transcription elongation. We demonstrate that the conserved
Saccharomyces cerevisiae
histone chaperone Nap1 associates with chromatin. We show that Nap1 regulates transcription of
PHO5
, and the increase in transcript level and the higher phosphatase activity plateau observed for Δ
nap1
cells suggest that the net function of Nap1 is to facilitate nucleosome reassembly during transcription elongation. To further our understanding of histone chaperones in transcription elongation, we identified factors that regulate the function of Nap1 in this process. One factor investigated is an essential mRNA export and TREX complex component, Yra1. Nap1 interacts directly with Yra1 and genetically with other TREX complex components and the mRNA export factor Mex67. Additionally, we show that the recruitment of Nap1 to the coding region of actively transcribed genes is Yra1 dependent and that its recruitment to promoters is TREX complex independent. These observations suggest that Nap1 functions provide a new connection between transcription elongation, chromatin assembly, and messenger RNP complex biogenesis.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Reference57 articles.
1. Abruzzi, K. C., S. Lacadie, and M. Rosbash. 2004. Biochemical analysis of TREX complex recruitment to intronless and intron-containing yeast genes. EMBO J.23:2620-2631.
2. Adkins, M. W., S. R. Howar, and J. K. Tyler. 2004. Chromatin disassembly mediated by the histone chaperone Asf1 is essential for transcriptional activation of the yeast PHO5 and PHO8 genes. Mol. Cell14:657-666.
3. Adkins, M. W., and J. K. Tyler. 2006. Transcriptional activators are dispensable for transcription in the absence of Spt6-mediated chromatin reassembly of promoter regions. Mol. Cell21:405-416.
4. Aguilera, A. 2005. Cotranscriptional mRNP assembly: from the DNA to the nuclear pore. Curr. Opin. Cell Biol.17:242-250.
5. Aparicio, O. 1999. Characterization of proteins bound to chromatin by immunoprecipitation from whole-cell extracts, p. 21.3.1-21.3.12. In R. B. F. M. Ausubel, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.), Current protocols in molecular biology, vol. 4. John Wiley and Sons, Inc., New York, NY.
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