Author:
Sack D A,Huda S,Neogi P K,Daniel R R,Spira W M
Abstract
We have developed a microtiter enzyme-linked immunosorbent assay method for detecting the heat-labile enterotoxins of Vibrio cholerae and Escherichia coli using GM1 ganglioside as the base coat. This method compares favorably with a similar assay using anticholera toxin as the base coat, and with the Y1 adrenal cell assay. The assay should be useful in detecting enterotoxin production in E. coli and vibrios (including non-agglutinating Vibrio), in quantitating the toxin, and in determining binding properties of enterotoxins to ganglioside. The assay can also be used to quantitate antibodies which block the attachment of the toxin to the ganglioside.
Publisher
American Society for Microbiology
Reference14 articles.
1. Adaptation of staphylococcal-coagglutination technique for detection of heat-labile enterotoxin of Escherichia coli;Brill B. M.;J. Clin. Microbiol.,1979
2. Preparation of the vascular permeability factor of Vibrio cholerae;Craig J. P.;J. Bacteriol.,1966
3. Direct serological assay for the heat-labile enterotoxin of Escherichia coli, using passive immune hemolysis;Evans D. J.;Infect. Immun.,1977
4. Development and standardization of a cytotoxic microassay for detection of enterotoxins: survey of enterotoxins from Escherichia coli of infant origin;Farkas-Himsley H.;Microbios,1977
5. Feeley J. E. S. Beck J. P. Craig C. Hardegree C. E. Miller R. Northrup and E. Seligmann. 1976. Cholera antitoxin: report of an ad hoc committee U.S. Cholera Panel. Proceedings of the 12th Joint Conference U.S.-Japan Cooperative Medical Science Program Cholera Panel Sapporo.
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