The OprF porin as a potential target for the restoration of carbapenem susceptibility in <i>Pseudomonas aeruginosa</i> expressing acquired carbapenemases-Reference-Cited by-同舟云学术

The OprF porin as a potential target for the restoration of carbapenem susceptibility in Pseudomonas aeruginosa expressing acquired carbapenemases

Published:2024-09-12 Issue: Volume: Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Nordmann Patrice¹²³⁴^ORCID,Helsens Nicolas¹⁵^ORCID,Poirel Laurent¹²³⁴^ORCID,Sadek Mustafa³^ORCID,Bumann Dirk⁶,Findlay Jacqueline³^ORCID

Affiliation:

1. European Institute for Emerging Antibiotic Resistance, Pasteur Institute, Lille, France

2. European Institute for Emerging Antibiotic Resistance, University of Fribourg, Fribourg, Switzerland

3. Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, Switzerland

4. Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland

5. Clinical Microbiology Unit, Pasteur Institute of Lille, Lille, France

6. Biozentrum, University of Basel, Basel, Switzerland

Abstract

ABSTRACT Carbapenem resistance in Pseudomonas aeruginosa is primarily due to the acquisition of carbapenemases and is often associated with a diminution of the membrane permeability. The outer membrane protein, OprD, is a well-known route, by which carbapenems, predominantly imipenem, can enter the cell, and its loss has been associated with reduced susceptibility to imipenem. In this study, we investigated the antibiotic susceptibility patterns of isogenic P. aeruginosa mutants containing various acquired β-lactamases, including carbapenemases, in a porin-depleted background. We identified that the deletion of oprF was associated with some recovery of susceptibility to carbapenems.

Publisher

American Society for Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/aac.00761-24

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