Detection of Coxiella burnetii DNA in Inhalable Airborne Dust Samples from Goat Farms after Mandatory Culling

Author:

Hogerwerf Lenny1,Borlée Floor2,Still Kelly2,Heederik Dick2,van Rotterdam Bart3,de Bruin Arnout3,Nielen Mirjam1,Wouters Inge M.2

Affiliation:

1. Faculty of Veterinary Medicine, Department of Farm Animal Health, Utrecht, the Netherlands

2. Institute for Risk Assessment Sciences (IRAS), Division of Environmental Epidemiology, Utrecht, the Netherlands

3. National Institute for Public Health and the Environment (RIVM), Laboratory for Zoonoses and Environmental Microbiology, Bilthoven, the Netherlands

Abstract

ABSTRACT Coxiella burnetii is thought to infect humans primarily via airborne transmission. However, air measurements of C. burnetii are sparse. We detected C. burnetii DNA in inhalable and PM10 (particulate matter with an aerodynamic size of 10 μm or less) dust samples collected at three affected goat farms, demonstrating that low levels of C. burnetii DNA are present in inhalable size fractions.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference14 articles.

1. Q fever;Angelakis E;Vet. Microbiol.,2010

2. Coxiella burnetii shedding and environmental contamination at lambing in two highly naturally-infected dairy sheep flocks after vaccination

3. de BruinA. 2011. Q fever: the answer is blowing in the wind. Detection of Coxiella burnetii in aerosols. RIVM rapport 330291005. National Institute for Public Health and the Environment Bilthoven Netherlands. http://www.rivm.nl/bibliotheek/rapporten/330291005.pdf.

4. Detection of Coxiella burnetii in Complex Matrices by Using Multiplex Quantitative PCR during a Major Q Fever Outbreak in The Netherlands

5. Detection of Coxiella burnetii DNA on Small-Ruminant Farms during a Q Fever Outbreak in the Netherlands

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