Affiliation:
1. Dipartimento di Medicina Sperimentale e Patologia1 and
2. Istituto di Virologia,2 Università di Roma “La Sapienza,” Rome, and
3. Istituto Neurologico Mediterraneo “Neuromed,” Pozzilli,3 Italy
Abstract
ABSTRACT
Ultrastructural analysis of HSB-2 T-lymphoid cells and human cord blood mononuclear cells infected with human herpesvirus 6 revealed the presence, in the cell cytoplasm, of annulate lamellae (AL), which were absent in uninfected cells. Time course analysis of the appearance of AL following viral infection showed that no AL were visible within the first 72 h postinfection and that their formation correlated with the expression of the late viral glycoprotein gp116. The requirement of active viral replication for AL neoformation was further confirmed by experiments using inactivated virus or performed in presence of the viral DNA polymerase inhibitor phosphonoacetic acid. Both conventional electron microscopic examination and immunogold fracture labeling with anti-endoplasmic reticulum antibodies indicated a close relationship of AL with the endoplasmic reticulum and nuclear membranes. However, when the freeze-fractured cells were immunogold labeled with an anti-gp116 monoclonal antibody, AL membranes were densely labeled, whereas nuclear membranes and endoplasmic reticulum cisternae appeared virtually unlabeled, showing that viral envelope glycoproteins selectively accumulate in AL. In addition, gold labeling with
Helix pomatia
lectin and wheat germ agglutinin indicated that AL cisternae, similar to
cis
-Golgi membranes, contain intermediate, but not terminal, forms of glycoconjugates. Taken together, these results suggest that in this cell-virus system, AL function as a viral glycoprotein storage compartment and as a putative site of O-glycosylation.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference43 articles.
1. Pores of annulate lamellae and nuclei bind wheat germ agglutinin and monoclonal antibody similarly;Allen E. D.;J. Struct. Biol.,1990
2. Identification of proteins specific for human herpesvirus 6-infected human T cells
3. Proteins of human herpesvirus 6;Balachandran N.;Perspect. Med. Virol.,1992
4. The interaction of wheat germ agglutinin with sialoglycoproteins. The role of sialic acid;Bhavandan V. P.;J. Biol. Chem.,1979
5. Ultrastructural characterization of a new human B lymphotropic DNA virus (human herpesvirus 6) isolated from patients with lymphoproliferative disease;Biberfeld P.;J. Natl. Cancer Inst.,1987
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