Lysin LysMK34 of Acinetobacter baumannii Bacteriophage PMK34 Has a Turgor Pressure-Dependent Intrinsic Antibacterial Activity and Reverts Colistin Resistance-Reference-Cited by-同舟云学术

Lysin LysMK34 of Acinetobacter baumannii Bacteriophage PMK34 Has a Turgor Pressure-Dependent Intrinsic Antibacterial Activity and Reverts Colistin Resistance

Published:2020-09-17 Issue:19 Volume:86 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Abdelkader Karim¹²^ORCID,Gutiérrez Diana¹^ORCID,Grimon Dennis¹,Ruas-Madiedo Patricia³^ORCID,Lood Cédric⁴⁵^ORCID,Lavigne Rob⁴^ORCID,Safaan Amal⁶,Khairalla Ahmed S.²⁷^ORCID,Gaber Yasser²⁸^ORCID,Dishisha Tarek²^ORCID,Briers Yves¹^ORCID

Affiliation:

1. Department of Biotechnology, Ghent University, Ghent, Belgium

2. Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt

3. Dairy Research Institute of Asturias, Spanish National Research Council (IPLA-CSIC), Villaviciosa, Asturias, Spain

4. Department of Biosystems, KU Leuven, Leuven, Belgium

5. Department of Microbial and Molecular Systems, Centre of Microbial and Plant Genetics, Laboratory of Computational Systems Biology, KU Leuven, Leuven, Belgium

6. Department of Microbiology and Immunology, Faculty of Pharmacy, Menoufia University, Shebin ElKoum, Egypt

7. Department of Biology, University of Regina, Regina, Saskatchewan, Canada

8. Department of Pharmaceutics and Pharmaceutical Technology, College of Pharmacy, Mutah University, Karak, Jordan

Abstract

A. baumannii is one of the most challenging pathogens for which development of new and effective antimicrobials is urgently needed. Colistin is a last-resort antibiotic, and even colistin-resistant A. baumannii strains exist. Here, we present a lysin that sensitizes A. baumannii for colistin and can revert colistin resistance to colistin susceptibility. The lysin also shows a strong, turgor pressure-dependent intrinsic antibacterial activity, providing new insights in the mode of action of lysins with intrinsic activity against Gram-negative bacteria.

Funder

FWO Vlaanderen

Cultural Affairs and Missions Sector, Ministry of Higher Education

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01311-20

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