Acetate and Acetyl-CoA Metabolism of ANME-2 Anaerobic Archaeal Methanotrophs-Reference-Cited by-同舟云学术

Acetate and Acetyl-CoA Metabolism of ANME-2 Anaerobic Archaeal Methanotrophs

Published:2023-06-28 Issue:6 Volume:89 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Ouboter Heleen T.¹²,Arshad Arslan¹,Berger Stefanie¹²,Saucedo Sanchez Jesus Gerardo¹,Op den Camp Huub J. M.¹^ORCID,Jetten Mike S. M.¹²^ORCID,Welte Cornelia U.¹²^ORCID,Kurth Julia M.¹²³^ORCID

Affiliation:

1. Radboud Institute of Biological and Environmental Sciences, Microbiology Cluster, Radboud University, Nijmegen, Netherlands

2. Soehngen Institute of Anaerobic Microbiology, Nijmegen, Netherlands

3. Microcosm Earth Center, Philipps-Universität Marburg and Max Planck Institute for Terrestrial Microbiology, Marburg, Germany

Abstract

Acetyl-CoA plays a key role in carbon metabolism and is found at the junction of many anabolic and catabolic reactions. This work describes the biochemical properties of ACS and ACD enzymes from ANME-2 archaea. This adds to our knowledge of archaeal ACS and ACD enzymes, only a few of which have been characterized to date. Furthermore, we validated the in situ activity of ACS in ANME-2d, showing the conversion of acetate into methane by an enrichment culture dominated by ANME-2d.

Funder

Nederlandse Organisatie voor Wetenschappelijk Onderzoek

Soehngen Institute of Anaerobic Microbiology

EC | European Research Council

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.00367-23

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1. Methane Feedbacks to the Global Climate System in a Warmer World